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ISSN : 1226-7155(Print)
ISSN : 2287-6618(Online)
International Journal of Oral Biology Vol.31 No.2 pp.45-51

Molecular Characterization of Cαv23 in Rat Trigeminal Ganglion Neurons

Seog-Bae Oh, Kim Joong-Soo, Fang Zhi
Department of Physiology and Program in Molecular and Cellular Neuroscience College of Dentistry and Dental Research Institute Seoul National University


R-type(Cαv23) calcium channel contributes to pain sensation in peripheral sensory neurons. Six isoforms of Cαv23 that result from combinations of presence or deletion of three inserts(insert I and insert in the II-III loop, and insert III in N-terminal regions) have been demonstrated to be present in different mammalian tissues. However, the molecular basis of Cαv23 in trigeminal ganglion(TG) neurons is not known. In the present study, we determined which isoforms of Cαv23 are expressed in rat TG neurons using the RT-PCR analysis. Whole tissue RT-PCR analyses revealed that only two isoforms, Cαv23α and Cαv23e, were present in TG neurons. From single-cell RT-PCR, we found that Cαv23e rather than Cαv23α was the major isoform expressed in TG neurons, and Cαv23e was preferentially detected in small-sized neurons that express nociceptive marker, transient receptor potential vanilloid 1(TRPV1). Our results suggest that Cαv23e in trigeminal neurons may be a potential target for the pain treatment.