Von Ebner's glands (vEG) are minor salivary glands associated with circumvallate and foliate papilla. The secretions of vEG consist of microenvironment of the taste buds in the circumvallate and foliate papillae, and thus saliva from vEG plays a role in the perception of taste. The Cα²⁺ signaling system in rat vEG acinar cell was examined using the Cα²⁺-sensitive fluorescent indicator Fura-2. Agonist-induced increase in intracellularCα²⁺([Cα²+]_iwas stimulated by carbachol (CCh) and substance P (SP), but not by norepinephrine (NE), and recovered to control levels by their receptor antagonists dose-dependently. The effects were also observed in Cα²⁺-free medium, suggesting mobilization from intracellular Cα²⁺ store. These results in the vEG acinar cell indicate that 1) [Cα²⁺]_i is at least regulated by muscarinic and neurokininergic (NK1) receptors; 2) the increases in [Cα²⁺]_i activated by CCh and SP are mainly mediated by discharge of cytosolic calcium pool.