Antimicrobial Effects of Ursolic Acid against Mutans Streptococci Isolated from Koreans

1Joong-Ki Kook, 1Min Jung Kim, 1Chun Sung Kim, 2Jae-Yoon Park, 1Yun Kyong Lim, 1Soon-Nang Park, 3Sug-Joon Ahn, 4Dong-Chun Jin, 5Tae Hyung Kim

1Department of Oral Biochemistry and Oral Biology Research Institute, School of Dentistry,Chosun University
2Department of Biochemistry and Molecular Biology, Medical School, Chosun University,
3Dental Research Institute and Department of Orthodontics, College of Dentistry, Seoul National University,
4Department of Veterinary Medicine, College of Agriculture, Yanbian University
5Department of Restorative Sciences, Herman Ostrow School of Dentistry, University of Southern California

(received December 13, 2010 ; revised January 28, 2011 ; accepted February 11, 2011)

Abstract

Ursolic acid is a triterpenoid compound present in manyplants. This study examined the antimicrobial activity ofursolic acid against mutans streptococci (MS) isolated fromthe Korean population. The antimicrobial activity wasevaluated by the minimum inhibitory concentration (MIC)and time kill curves of MS. The cytotoxicity of ursolic acidagainst KB cells was tested using an MTT assay. The MIC90values of ursolic acid for Streptococcus mutans andStreptococcus sobrinus isolated from the Korean populationwere 2 μg/ml and 4 μg/ml, respectively. Ursolic acid had abactericidal effect on S. mutans ATCC 25175Tand S.sobrinus ATCC 33478Tat > 2 × MIC (4 μg/ml) and 4 × MIC(8 μg/ml), respectively. Ursolic acid had no cytotoxic effecton KB cells at concentrations at which it exertedantimicrobial effects. The results suggest that ursolic acidcan be used in the development of oral hygiene products forthe prevention of dental caries.Key words: antimicrobial effect,

Key Words : antimicrobial effect; Streptococcus mutans; Streptococcus sobrinus; ursolic acid

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Antimicrobial Effects of Ursolic Acid against Mutans Streptococci Isolated from Koreans

Abstract

Reference