Voltage dependent calcium channel (VDCC), one of themost important regulator of Ca2+concentration in neuron,play an essential role in the central processing of nociceptiveinformation. The present study investigated the antinociceptiveeffects of L, T or N type VDCC blockers on theformalin-induced orofacial inflammatory pain. Experimentswere carried out on adult male Sprague-Dawley ratsweighing 220-280 g. Anesthetized rats were individuallyfixed on a stereotaxic frame and a polyethylene (PE) tube wasimplanted for intracisternal injection. After 72 hours, 5%formalin (50 μL) was applied subcutaneously to the vibrissapad and nociceptive scratching behavior was recorded fornine successive 5 min intervals. VDCC blockers wereadministered intracisternally 20 minutes prior to subcutaneousinjection of formalin into the orofacial area. Theintracisternal administration of 350 or 700 μg of verapamil, ablocker of L type VDCC, significantly decreased the numberof scratches and duration in the behavioral responsesproduced by formalin injection. Intracisternal administrationof 75 or 150 μg of mibefradil, a T type VDCC blocker, or11 or 22 μg of cilnidipine, a N type VDCC blocker, alsoproduced significant suppression of the number of scratchesand duration of scratching in the first and second phase.Neither intracisternal administration of all VDCC blockersnor vehicle did not affect in motor dysfunction. The presentresults suggest that central VDCCs play an important role inorofacial nociceptive transmission and a targeted inhibitionof the VDCCs is a potentially important treatment approachfor inflammatory pain originating in the orofacial area.